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Membrane proteins are frequently the targets for binding ligands, including small drug molecules. The intrinsic chirality of protein drug receptors often governs specific pharmacological response to ligands with particular stereochemical geometries. Characterization of the potency, efficacy, and safety of individual enantiomeric ligands is therefore important. Naturally occurring cannabinoids often contain chiral molecules, and therefore stereoselective analysis of phytocannabinoids may prove essential for studying efficacy, phenotype determination, and quality control and stability testing of medicinal cannabis. Chiral analysis is even more critical for synthetically-produced cannabinoids, as creating stereoisomers in the synthesis process is often unavoidable. We demonstrate chromatographic resolution of the natural racemic cannabinoid, cannabichromene (CBC) , in plant extract, along with the synthetic racemic (+/-)-Δ 9 -trans-tetrahydrocannabinol using supercritical fluid chromatography (SFC) coupled with mass spectrometry (MS). Download |